Overview
The Faculty of Medicine's Flow Cytometry Core Facility offers instrumentation as well as scientific expertise to perform multiparametric flow cytometry assays, and sorting of cells/particles. Our services include technical support, flow cytometry panel design, protocol optimization, consultations, data analysis and interpretation assistance, and training sessions available upon request.
Basic applications include:
- Immunophenotyping
- Viability and cell death assays
- Study of immune response dynamics (such as immune cells' activation, proliferation, cytokine production, memory)
- The BD FACSAria III Sorter also allows for the:
- Isolation of cellular subtypes
- Assessment of cellular morphology and internal components
- Tracking of intracellular changes (such as calcium influx and ROS generation)
- Elucidation of cellular signaling pathways