Bio-guided fractionation, Isolation & structure identification of an anti-inflammatory guaianolids from Centaurea ainetensis

​​Saliba, N.A., Dakdouki, S., Homeidan, F., Kogan, J., Bouhadir, K., Talhouk, S. and Talhouk, R. Bio-guided fractionation, Isolation & structure identification of an anti-inflammatory guaianolids from Centaurea ainetensis. Pharmaceutical Biology 2009 47:701-707.

Abstract

Inflammatory processes are characterized by the stimulation of humoral and cellular mediator systems resulting in increased levels of mediators of inflammation such as interleukins (ILs) and prostaglandins (PGs), which play central roles in regulating the inflammatory response and inflammation-mediated damage. The aim of the study was to develop natural therapeutics for the interventions and/or prevention of inflammatory disorders. A methanol plant extract of Centaurea ainetensis Boiss. (Asteraceae) was tested for its anti-inflammatory potential activity in two cell models of inflammation, which served as the basis for the bio-guided fractionation of the extract and the identification of its active ingredient salograviolide A. The guaianolide salograviolide inhibited endotoxin (ET)-induced IL-6 levels in SCp2 mammary epithelial cells and decreased the levels of IL-1-induced cyclooxygenase enzyme levels in intestinal epithelial cells. The results provide evidence for the anti-inflammatory activities of the sesquiterpene agent salograviolide A and the biochemical basis for further studies on salograviolide A mechanism of action.